| Other Notes | The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform. |
| | For additional information, please see www.sigma-aldrich.com/post-reaction. |
| Features and Benefits | • Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
• Recovers up to 95% of PCR products between 100 bp and 10 kb
• Removes over 99% of primers and other components
• Eliminates the need to remove mineral oil by organic extraction
• 40% more purification preps supplied than market leader
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| Principle | DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μl or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing (Fig. 2), cloning and microarray analysis. |
| Legal Information | GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
